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ID 32024
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 1.49 MB
著者
Ono, Toshiaki Okayama University
Miyaishi, Satoru Okayama University
Yamamoto, Yuji Okayama University
Yoshitome, Kei Okayama University
Ishikawa, Takaki Okayama University
Ishizu, Hideo Okayama University
抄録

We developed a method for human identification of forensic biological materials by PCR-based detection of a human-specific sequence in exon 3 of the myoglobin gene. This human-specific DNA sequence was deduced from differences in the amino acid sequences of myoglobins between humans and other animal species. The new method enabled amplification of the target DNA fragment from 30 samples of human DNA, and the amplified sequences were identical with that already reported. Using this method, we were able to distinguish human samples from those of 21 kinds of animals: the crab-eating monkey, horse, cow, sheep, goat, pig, wild boar, dog, raccoon dog, cat, rabbit, guinea pig, hamster, rat, mouse, whale, chicken, pigeon, turtle, frog, and tuna. However, we were unable to distinguish between human and gorilla samples. This method enabled us to detect the target sequence from 25 pg of human DNA, and the target DNA fragment from blood stored at 37 degrees C for 6 months, and from bloodstains heated at 150 degrees C for 4 h or stored at room temperature for 26 years. Herein we also report a practical application of the method for human identification of a bone fragment.</P>

キーワード
species identification
myoglobin
polymerase chain reaction
Amo Type
Article
発行日
2001-06
出版物タイトル
Acta Medica Okayama
55巻
3号
出版者
Okayama University Medical School
開始ページ
175
終了ページ
184
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT