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ID 31971
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 1.87 MB
著者
Imabayashi, Kiyomi Okayama University
Yamamoto, Yuji Okayama University
Inagaki, Sachiyo Okayama University
Doi, Yusuke Okayama Prefectural Police Headquarters
Yoshitome, Kei Okayama University
Miyaishi, Satoru Okayama University
Ishizu, Hideo Okayama University
抄録

We have improved on conventional methods for HLA-DRB1 genotyping and devised a new method that is simple, cost-effective, and adequately applicable to routine forensic practice. This method consists of group-specific polymerase chain reaction (PCR) of the exon 2 region of the HLA-DRB1 gene and simultaneous detection of single nucleotide polymorphisms (SNPs) at multiple sites using multiplex primer extension reactions. With this method, we successfully detected HLA-DRB1 genotypes from the following materials: the peripheral blood of 142 donors, 6 aged saliva stains of known DRB1 genotype stored for 5-10 years at room temperature, 10 aged bloodstains of unknown DRB1 genotype stored for 29 years at room temperature, and minimal bloodstains and saliva stains from 3 donors of known DRB1 genotypes. Furthermore, we were able to type DRB1 alleles of the minor component in mixed samples at a proportion of 1/1,000 or 1/10,000. In a criminal case, DRB1 alleles detected from mixed bloodstains on a sword found at the scene enabled us to explain the case. This method is expected to be useful for forensic medicine.

キーワード
HLA-DRB1 genotyping
group specific primer
single nucleotide polymorphism
multiplex primer extension reactions
application to mixed samples
Amo Type
Article
発行日
2005-10
出版物タイトル
Acta Medica Okayama
59巻
5号
出版者
Okayama University Medical School
開始ページ
179
終了ページ
194
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT