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ID 16645
Eprint ID
16645
フルテキストURL
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タイトル(別表記)
Metallic mercury uptake by catalase In Vitro metallic mercury uptake by rat liver dissociated cells, homogenate, cell components, heme protein and ferric ion, with the effect of ethyl and methyl alcohol on the uptake
著者
劒持 堅志 岡山大学医学部公衆衛生学教室
抄録
In Vitro metallic mercury uptake by rat liver dissociated cells, homogenate, cell components (microbody-rich fraction, mitochondrial fraction and microsomal fraction), heme protein (catalase, cytochrome c, methemoglobin and hematine) and ferric ion were investigated. The effects of ethyl and methyl alcohol on the uptake were also investigated. The following results were obtained. 1) Mercury uptake by rat liver dissociated celld was similar to the uptake by homogenate. 2) Mercury uptake by rat liver homogenate in the presence of hydrogen peroxide was 1.22 times the uptake in the absence of hydrogen peroxide. The uptake by rat liver dissociated cells in the presence of hydrogen peroxide was similar to the uptake in the absence of hydrogen peroxide. 3) Mercury uptake by crystalline catalase, rat liver dissociated cells and homogenate was inhibited by ethtl alcohol and methyl alcohol, though inhibition of catalase activities was not observed. 4) Mercury uptake by rat liver cell components was observed in the order of microbody-rich fraction > microsomal fraction > mitochondrial fraction, and catalase activities were also observed in the order of microbody-rich fraction > microsomal fraction > mitochondrial fraction. Mercury uptake by rat liver cell components was inhibited by potassium cyanide and azide. 5) Cytochrome c, methemoglobin, catalase and ferric ion oxidized metallic mercury in the presence of hydrogen peroxide. In the absence of hydrogen peroxide, methemoglobin and ferric ion oxidized, but catalase and cytochrome c did not oxidize metallic mercury. 6) Hematine did not oxidize metallic mercury with or without hydrogen peroxide. 7) Uptake by ferric ion was inhibited by equimolar addition of EDTA. 8) In the presence of hydrogen peroxide, mercury uptake by catalase was 27,000 times, cytochrome c was 140 times and methemoglobin was 35 times the uptake by ferric ion. Therefore, of heme protein catalase showed the highest mercury uptake.
キーワード
Catalase
Cytochrom C
Methemoglobin
Cell Components
Dissociated Cell
発行日
1981-10-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
93巻
9-10号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
835
終了ページ
843
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/93/9-10/93_9-10_835/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma