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ID 40717
フルテキストURL
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タイトル(別表記)
Immunohistochemical studies on the binding of lectin (PNA) and human anti-liver cell membrane antibody to the hepatic carcinoma cell membrane of rats fed 3'-methyl-4-dimethylaminoazobenzene (DAB)
著者
冨田 治 岡山大学医学部第一内科学教室
抄録
Membrane changes of DAB hepatic carcinoma cells were examined immunohistochemically, using the binding activity of Arachis hypogaea agglutinin (PNA) and anti-liver membrane antibody (anti-LM) from patients with HBsAg-negative chronic active hepatitis. The hepatic carcinoma in rats was produced through DAB feedings for 3-5 months. As controls, non-cancerous liver tissues from rats fed DAB, and normal rat liver were used. In isolated calls of the hepatic carcinoma and control tissues, FITC-PNA binded in 10 of 11 cases of DAB hepatic carcinoma and in none of control cases (11 rats fed DAB and 2 normal rats). In tissue sections, similar results were obtained. Lens culnaris agglutinin did not show any difference in binding among the 3 groups. Two anti-LMs did not bind to the membranes of isolated calls in 3 of 10 DAB hepatic carcinomas, although the anti-LMs were bound in all of controls. These facts indicate that the transformation, DAB hepatic carcinoma cells is accompanied by changes in the cell membrane, and that PNA may be useful in the analysis of membrane changes in carcinogenesis.
キーワード
DAB肝癌
PNA
肝細胞膜抗体免疫蛍光法
レクチン蛍光法
発行日
1989-02
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
101巻
1-2号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
97
終了ページ
105
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/101/1-2/101_1-2_97/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma