岡山医学会 Acta Medica Okayama 0030-1558 70 6 1958 家兎骨髄組織液体培養法による鉄, 銅及びコバルトの増血作用に関する研究 第2編 銅及びコバルトの増血作用 2205 2221 EN Katsuya Kumeda 1. Hematopoietic actions of cobalt and copper, either singly or in combination with iron have been studied by means of rabbit bone marrow culture in fluid medium. 2. When various cobalt compounds are added singly, with exception of cobalt chloride, every one of them acts markedly to help erythropoiesis, but Hb content on the contrary often decreases by such addition. Consequently, this hypochrome erythropoiesis seems to be due to lack of iron supply. 3. In the case where ferric gluconate is added in combination with cobalt compounds, Hb increses markedly, especially so when the combination is ferric gluconate and cobalt chloride in proportion of 1:50; suggesting that cobalt greatly assists the induction of iron into the heme nucleus. 4. Due to its toxicity, copper addition either singly or in combination with iron does not help erythropoiesis to any great extent, but when in combination with iron as in the case of cobalt copper helps to increase Hb content. 5. The addition of three metals, iron, copper, and cobalt, does not yield the best result, indicating the necessity of giving a consideration to the toxicity arising out of the amount to added. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 70 6 1958 家兎骨髄組織液体培養法による鉄, 銅及びコバルトの増血作用に関する研究 第1編 方法論及び鉄の増血作用 2191 2204 EN Katsuya Kumeda The author studied various conventional methods of bone marrow tissue culture in fluid medium and its history, and compared these methods with a modified form of own design, and obtained the following results: 1. With this modified method a series of rabbit bone marrow tissue culture in fluid medium has been performed and studied hematopoietic effects of inorganic ferrous and ferric compounds and organic ferric compounds by adding each of these compounds to the medium. As the results it has been found that ferric gluconate proves to be the most effective hematopoietic, moreover, when this compound is added to the medium along with serum, Hb content increases strikingly, thus indicating that serum is an indispensable factor Hem synthesis. 2. Among iron compounds other than ferric gluconate no appreciable difference can be found in their hematolwietic effectiveness, at least from the standpoint of its direct action in bone marrow, it is not the valency of iron itself nor organic or inorganic nature, but rather the form of the compounds, that will have any bearing on the utilization of iron for the production of Hb. No potential conflict of interest relevant to this article was reported.