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ID 17655
Eprint ID
17655
フルテキストURL
Thumnail 88_737.pdf 1.25 MB
タイトル(別表記)
Experimental study on the combined effect of anticancer agent and urokinase in the autotransplantation of autochthonous tumor of mice
著者
中嶋 健博 岡山大学医学部第二外科教室
抄録
The tumor, induced by intramuscular inoculation of methylcholanthrene in the thigh of mice, was removed by amputation of the leg and the cell was isolated by mincing and trypsinizing the tumor fragment, and suspended in isotonic saline in counted number after viability was tested by trypan blue dye stayning. Mitomycin C (MMC) and Urokinase (UK) was administered either in vitro or in vivo and an effect was evaluated in the transplantability of the tumor cell which was inoculated in the subcutaneous tissue of the back of their own hosts. The results were summarized as follows: 1) Only when the tumor cell was inoculated with MMC, the concentration of which was minimally enough to lower the transplantability by itself, the addition of UK suppressed the take of the tumor, slightly more than that in MMC treatment. No evidence was obtained that the concentration of MMC below the above gained cytostatic effect by the addition of UK in vitro. 2) Definite suppression of take of the transplanted tumor cell and marked prolongation of interval between the transplantation and take were observed, when the combined intraperitoneal administration of MMC and UK was started in the day of the transplantation, compaired with that in mice MMC alone was samely given. The growth of the tumor was retarded in accordance with this suppression in the tumor take. It was also observed that the take of the tumor cell was diminished to some extent even in mice treated with UK alone. However, when the treatment was started 10 days after the transplantation, the growth of established tumors was not affected by the combined application of UK. It was concluded that adjuvant effect of UK in anticancer chemotherapy for prevention of tumor metastasis could be expected and an assumption was made that the mechanism lie in interruption of tumor cell lodgement through fibrinolysis caused by UK administration.
発行日
1976-10-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
88巻
9-10号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
737
終了ページ
758
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/88/9-10/88_9-10_737/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma