Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

An Endonuclease Excising 8-Oxo-2'-deoxyguanosine in Regenerating Rat Liver

田原 正一 Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology
田口 隆彦 Department of Gene Regulation and Protein Function, Tokyo Metropolitan Institute of Gerontology
金子 孝夫 Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology
馬場 直道 岡山大学
抄録
Oxidative DNA damage is generated in every tissue in the body, and is mainly excised by glycosylases. However, endonucleases and exonucleases are suggested as being present in tissues, since oxidized nucleosides such as thymidine glycol and 8-Oxo-2'-deoxyguanosine are detected in urine. For this reason, we studied repair enzymes induced in regenerating rat liver, and detected an enzyme cleaves phosphodiester bonds on the 5'side of 8-oxo-2'-deoxyguanosine nucleotides in DNA. The coexistence of this enzyme and phosphodiesterase II results in the release of 8-oxo-2'-deoxyguanosine monophosphate from calf thymus DNA enriched with 8-oxoguanine by γ-ray irradiation. This enzyme is found in regenerating rat liver, but not in normal rat liver. The enzyme may have a specific connection to DNA replication.
キーワード
oxidative DNA damage
repair
endonuclease
8-oxo-2'-deoxyguanosine
ISSN
0474-0254
NCID
AN00033029