Proceedings of Okayama Association for Laboratory Animal Science
Published by Okayama Association for Laboratory Animal Science

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工藤 季之 就実大学
Genome editing technology by the CRISPR/Cas9, which was developed in 2012, is applicable in a variety of species. In birds, because the techniques of gene transfer and gene disruption has not been established, CRISPR/Cas9 method using adeno-associated virus (AAV) vector is the ideal combination as genome editing in vivo. However, in the use of AAV vectors, there is a problem to be solved that there is a limit on the size of the gene can be introduced. Therefore, it is important to minimize as much as possible the size of the gene. One strategy is to change the Cas9 gene into smaller ones, the other one is to minimize the promoter sequence. In this study, for the purpose of minimization of the promoter sequence to be introduced, the activities of the promoters to be general-purpose in mammals were verified in avian cells. CMV, CAG, and miCMV promoter shorten the CMV are all had sufficient activity in avian cells.