The muscular dystrophy chicken has been studying as model animal of muscular dystrophy for more
than 50 years. Recently, the mutation of WW domain containing E3 ubiquitin protein ligase 1 (WWP1)
gene has been identified as a responsible for muscular dystrophy chicken. We observed that muscular
dystrophy chicken not only showed the degeneration of skeletal muscles but also produced watery feces.
Therefore, we examined the possibility of abnormalities in water metabolism of muscular dystrophy
chicken. We first analyzed plasma osmolality and gene expression of aquaporin 2 (AQP2), AQP3 and
alpha subunit of the amiloride-sensitive epithelial sodium channel (αENaC) in muscular dystrophy
chicken and White Leghorn chicken under normal physiological conditions at five-week old.
Subsequently, we analyzed these same parameters after one-day water-deprivation. The main findings of
our study are that: I) the plasma osmolality was significantly higher in muscular dystrophic chicken than
in White Leghorn; II) kidney αENaC mRNA expression was significantly lower in muscular dystrophic
chicken than in White Leghorn; III) AQP2 and AQP3 mRNA expressions in muscular dystrophic chicken
were similar in White Leghorn. We suggest that the mutation of WWP1 may cause the abnormality of
sodium absorption, and thus muscular dystrophic chicken become hypernatremic.
特別講演要旨 (Summary of Special Lecture)