Proceedings of Okayama Association for Laboratory Animal Science
Published by Okayama Association for Laboratory Animal Science

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筋ジストロフィーニワトリの水分代謝異常

齋藤 昇 岡山大学大学院環境生命科学研究科
抄録
The muscular dystrophy chicken has been studying as model animal of muscular dystrophy for more than 50 years. Recently, the mutation of WW domain containing E3 ubiquitin protein ligase 1 (WWP1) gene has been identified as a responsible for muscular dystrophy chicken. We observed that muscular dystrophy chicken not only showed the degeneration of skeletal muscles but also produced watery feces. Therefore, we examined the possibility of abnormalities in water metabolism of muscular dystrophy chicken. We first analyzed plasma osmolality and gene expression of aquaporin 2 (AQP2), AQP3 and alpha subunit of the amiloride-sensitive epithelial sodium channel (αENaC) in muscular dystrophy chicken and White Leghorn chicken under normal physiological conditions at five-week old. Subsequently, we analyzed these same parameters after one-day water-deprivation. The main findings of our study are that: I) the plasma osmolality was significantly higher in muscular dystrophic chicken than in White Leghorn; II) kidney αENaC mRNA expression was significantly lower in muscular dystrophic chicken than in White Leghorn; III) AQP2 and AQP3 mRNA expressions in muscular dystrophic chicken were similar in White Leghorn. We suggest that the mutation of WWP1 may cause the abnormality of sodium absorption, and thus muscular dystrophic chicken become hypernatremic.
備考
特別講演要旨 (Summary of Special Lecture)