Journal of Okayama Medical Association
Published by Okayama Medical Association

Full-text articles are available 3 years after publication.

肺癌関連モノクローナル抗体の免疫組織化学的解析 第1編 ヒトintermediate cell type肺小細胞癌細胞株(SBC-1)に対するモノクローナル抗体の作製と解析

中村 邦彦 岡山大学医学部第2内科
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Four IgM monoclonal antibodies against an intermediate cell type small cell lung cancer(SCLC) cell line(SBC-1) were produced and characterized. Reactivities of the antibodies to cell lines were tested by membrane immunofluorescence, and the reactions with normal and neoplasma tissues were analyzed using an indirect immunoperoxidase technique. Each of the 4 antibodies reacted with lung cancer cell lines and lung cancer tissues, but did not exhibit lung cancer histology-type specificity. The 4 antibodies reacted with normal bronchiolar and digestive gland tissues, and the antibody-reactive antigens were shown to be a gland-tissue differentiation antigen. The antigen was expressed on O type red cells, was not expressed on other erythrocytes and was undetectable in intermediate SCLC tissues. This may represent the epitope-difference of the antigen recognized by the antibodies. None of the antibodies reacted with oat SCLC, but intermediate and non-small lung cancer reacted with the antibodies. This result shows the antigen-similarity of intermediate SCLC with non-small lung cancer.
Small cell lung cancer
Monoclonal antibody
Immunohistochemical study