Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

培養グリオーマ細胞の形態学的分化に伴うDNAヒストグラム並びにS-100蛋白量の経時的変化に関する研究

古田 知久 岡山大学医学部脳神経外科学教室
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抄録
A flow cytometric analysis of DNA synthesis and a quantitative investigation of nervous system-specific S-100 protein were performed on cultured rat glioma (C-6) cells in the course of morphological differentiation induced by 1 mM dibutyryl cyclic AMP (dbc-AMP). The flow cytometric analysis revealed that a marked suppression of DNA synthesis was caused by dbc-AMP. The content of S-100 protein in dbc-AMP treated cells began to increase on the fourth day after the treatment with a subsequent increase to approximately 10-fold over the control cells at the stationary phase. These results suggest that S-100 protein is a useful biochemical index for estimating the degree of differentiation of glioma cells.
キーワード
培養グリオーマ細胞
S-100蛋白
micro-complement fixation assay
flow cytometry
DNA ヒストグラム
ISSN
0030-1558
NCID
AN00032489