Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

人白血球の試験管内簡易培養法について

俵 寿太郎 岡山大学医学部微生物学教室
寺坂 隆 岡山大学医学部微生物学教室
坂野 才蔵 岡山大学医学部微生物学教室
西井 怜 岡山大学医学部微生物学教室
柴田 醇 岡山大学医学部微生物学教室
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抄録
The normal healthy human blood was heparinized with one-tenth volume of 0.1 per cent heparin solution. Ten thousand units of penicillin and 10mg of streptomycin were added to each 100ml of the blood. The whole blood was distributed into culture tubes in amounts of 0.5ml or 1.0ml. The tubes were rubber-stoppered, inclined at 40~45 degrees against the level, and iucubated in static state at 37℃. A few days after incubation, the plasma and free blood cells in tubes were discarded and washed with PBS twice. Then fresh culture medium was placed into the tubes to maintain a number of cells remained adhered on the wall.
ISSN
0030-1558
NCID
AN00032489