Rabbits were immunized with Ehrlich ascitic tumor cells and then bled periodically. Following adsorption of the antisera with normal mice liver powder or packed Ehrlich tumor cells, the antisera was examined by electrophoresis, by anaerobic glycolysis by Warburg's manometry and for life prolongation study by neutralization. 1. In case of intravenously immunized rabbits sera, adsorbed γ-Globulin titre was comparably greater in the earlier stage with normal mice liver powder. Furthermore, the titre of γ-Globulin was greater and prolonged than the titre of preimmunization. 2. After adsorption with liver powder, the inhibitory action of antisera on anaerobic glycolysis of tumor cells was weak in the earlier stage, but still retained its former effectiveness in the later stage. 3. In the neutralization tests of adsorbed antisera, life prolongation was noted equally in the initial and later stages, with a decrease in the early period following adsorption. 4. Following adsorption of the antisera with the packed Ehrlich tumor cells, an ineffectiveness was revealed in both anaerobic glycolysis and neutralization. 5. It was deduced through points 2-4, that, the antisera produced in Part-1, immunized with Ehrlich tumor cells, contained antiumor antibody. 6. a) In IV immunized rabbits sera, adsorbed γ-Globulin titre was greater in the early stage. b) In anaerobic glycolysis and neutralization, the inhibitory action was weaker in the earlier stage. In supposition, the unadsorbed antisera produced from heterogenically and intravenously immunized animals contains species specific antibody chiefly in the earlier stage with tumor specific antibody chiefly in the later stage.