Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

人癌及びマウス癌組織の組織培養に関する研究 第2編 人子宮頸癌及びBashford氏マウス癌並びに正常マウス肝組織の組織培養に於ける糖代謝に就いて

白石 彰徳 岡山大学医学部平木内科教室
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抄録
The roller-tubo tissue cultures of human cervical carcinoma, Bashford's mouse carcinoma, and normal mouse liver tissue were performed in order to inquire into carbohydrate metabolism in the culture media. Glucose consumption, lactic acid, pyruvic acid, and α-ketoglutaric acid productions in cutlure media by human and animal tumors and by normal mouse liver tissue were estimated, and the following results have been obtained. 1. Glucose consumption and lactic acid production by human cervical carcinoma and Bashford's mouse carcinoma were in higher level than by normal mouse liver tissue. This was considered as the fact showing the high glycolysis of tumor tissues in vitro. 2. The ratios of lactic acid production/glcose consumption, pyruvic acid production/glucose consumption, α-ketoglutaric acid production/glueose consumption, in each tissue, were compared with each other. The ratio of lactic acid production/glucose consumption in every tissue turned out to be quite similar in values. But the ratios of pyruvic acid production/glucose consumption and α-ketoglutaric acid production/glucose consumption in Bashford's mouse carcinoma, were very low, about one third and one tenth of normal mouse liver tissue, respectively. In human cervical carcinoma, the ratio of pyruvic acid production/glucose consumption showed almost the same value as normal mouse liver tissue, but the ratio of α-ketoglutaric acid production/glucose consumption in human cervical carcinoma was half the value of normal mouse liver tissue. Thus pyruvic acid and α-ketoglutaric acid production, the components of tricarboyylic acid cycle, were reduced in tumor tissuen in vitro inspite of the same glucose consumption and lactic acid production as in normal mouse liver tissue in vitro. Therefore, the carbohydrate metabolism, as for the tricaroxylic acid cycle in tumor tissues, turned out to be different from normal mouse liver tissue. 3. The changes of hydrogen ion concentration in the culture media were in step with the course of tissue cultures. And the pH in tissue culture media had a correlation with the lactic acid production by tissues in vitro. But the increase in the pH was closely correlated with the lactic acid, pyruvic acid, and α-ketoglutaric acid productions by human cervical carcinoma, Bashford's mouse carcinoma, and normal mouse liver tissue in culture media.
ISSN
0030-1558
NCID
AN00032489