The change of hemoglobin in erythrocyte was observed on the action of l-ascorbic acid and molecular oxygen to erythrocyte, as same as the experiment in the first part and the hemolysis followed by the progression of reaction and the deficiency of washing condition of sediment were studied. And the results were as follows. 1. The reaction process on the action of 1-ascorbic acid and molecular oxygen to the hemolylic erythryocyte solution was antirely same to that on the action of 1-ascorbic acid and molecular oxygen to hemoglobin, but the reaction velocity was markedly prolonged. 2. In the upper clear solution 1. the reaction occuring on the action of 1-ascorbic acid and molecular oxygen in erythrocyte to a small amount of the hemolytic solution produced during the process was observed, but it was a little. 3. The formation of methemoglobin was only found in the upper clear solution 3, 4 on the aeration to reaction solution as same as the upper clear solution 2. 4. When the dosis of 1-ascorbic acid was not over the fixed dosis in comparison with the number of erythrocyte, it penetrated into erythrocyte and the product with the absorption maximum at 670 mμ was not produced and methemoglobin was only produced. 5. Choleglobin b was contained in the sediment 2. 6. When the change of hemoglobin was studied on the action of 1-ascorbic acid to erythrocyte, it was determined if methemoglobin was only produced, or the product with the absorption maximum at 670 mμ was also produced by the dosis of 1-ascorbic acid and it's acting condition.