Using the same procedure and organisms as in the Part I, the author prepared the soluble fraction and the particulate fraction, and carried out further enzymatic study of these fractions by means of Warburg's manometer techinipue. The following results were obtained. 1) The soluble fraction of H37 Rv and H37 Ra could oxidize lactate and other substrates fairly successfnlly; but on the other hand, the particulate fractions of that coule oxidize lactate and succinate specifically and failed to oxidize the other substrates. And this fact was also noticed in the case of the particulate fraction of BCG. 2) In the case of Takeo's strain one of the avian type bacilli, the enzyme system that oxidise malate was found merely in the particulate fraction. 3) It could be observed that the soluble fraction of Myc. ATCC 607 showed intense endogeneous respiration, and the particulate fraction of the organism could oxidize fumarate and malate specifically. 4) The soluble fraction of Myc. phlei could oxidize lactate successfully, but oxideze the other substrates not so satisfactorily. And in the case of the particulate fraction, it could oxidize any of lactate, succinate or malate, but, in a small extent.