Journal of Okayama Medical Association
Published by Okayama Medical Association

Full-text articles are available 3 years after publication.

骨髄組織培養に於ける顆粒細胞の固定,染色による研究 第I編 正常家兎,健康人胸骨骨髄組織被覆培養について

丹原 茂雄 岡山大学医学部平木内科教室
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In bone-marrow tissue culture of normal rabbits and of normal human sternum the author studied fixation and staining of the tissue in culture and also observed stain-findings of granulocytes and arrived at the following conclusions. 1. For the fixation and staining of the tissue in culture, the fixative of my own devise, namely, Zenker-glacial acetic acid-alcohol and Giemsa stain proved to give the least contraction of cells and to enable to differentiate bone-marrow granulocytes most readily. 2. The observations conducted from time to time by this method of fixation and staining give clearer pictures of the cell composition of cell growth area and bone-marrow granulocytes than vital observations. 3. Furthermore, by this method of observation it has been clarified that in bone-marrow tissue culture bone-marrow cells not only just wander out but also they mature and undergo cell division.