By adding polytamine, by-product of cow's milk hydrolysis and 10 different non-essential amino acids as already mentioned in Part 1 directly to rabbit bone-marrow tissue culture, the author studied influences of these amino acids on the wandering velocity of mature pseudoeosinophils in the bone marrow. 1. Polytamine and by-product of cow's milk caseous hydrolysis do not at all affect the wandering velocity of pseudoeosinophils. 2. Hydroxyproline, proline and cysteine, when added in a high concentration, all accelerate the wandering velocity but they do not at all affect the wandering velocity when added in a low concentration. Moreover, cysteine has been found to accelerate the wandering velocity even 12 hours after the start of culture just as much as at the third hour. The other two accelerate the wandering velocity markedly up to the sixth hour of culture. 3. Glutamic acid accelerates the wandering velocity slightly. 4. Arginine, serine, glycine, tyrodine, aspartic acid, and ornithine have neither accelerating action nor inhibitory action on the wandering velocity of pseudoeosinophils.