The Lugol solution was administrated and the changing of leukocytes, shock symptoms and skin reaction were studied every day after the prelimiary management. Thus the influence of Ravonal intravenous injection and cold anesthesia was researched. The results were as follows: 1) Injecting 0.03cc Lugol solution to normal guinea pigs each weighting 100g, shock symptoms appeared but using 0.01cc solution no general symptoms were found. 2) In normal guinea pigs, leukocytes decrease after the Lugol solution injection. Those which were managed preliminary by Lugol solution, leukocytes incresed in three or five days by the reinjection of Lugol solution. Even by injection of 0.03cc per 100g weight guinea pigs, they showed no shock symptoms and acquired tolerance. 3) On the 10th and 17th day from the preliminary management leukocytes decrease markedly if the reinjection of Lugol solution be done. Shock symptoms will be caused even by injection of 0.01cc per 100g weight guinea pigs and anaphylaxis found. 4) The skin reaction descends on the 3rd and the 5th day from the preliminary management (tolerance). On the 8th day it becomes nearly similar to normal guinea pigs and then gradually moves to anaphylaxis on the 11th and the 17th days. 5) For each reaction above mentioned the intravenous injection of Ravonal and cold anesthesia shows reduction. Only the control effects of Ravonal to the skin reaction is very slight. 6) The controlling mechanism of Ravonal and cold anesthesia for the general anaphylaxis is not the effect of the antigen and antibody combination nor that of the produced chemical reactive substances but is regarded to act on the decrease of reactive power of a living body.