Journal of Okayama Medical Association
Published by Okayama Medical Association

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Roller tube法による骨髄組織培養の吟味 第2編 鶏胎児圧搾液による影響について

菅野 卓 岡山大学医学部平木内科教室
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抄録
In the rabbit bone-marrow tissue culture by the roller-tube method, the author determined the optimal concentration in the medium of both the chick-embryo extracts and the first diluted solution from the sediment of the chick-embryo from which extracts was taken, and compared the effects of these two solutions on tissue culture. 1. As for the chick-embryo extracts, the relative tissue growth rate increases in a direct proportion to the concentration of the extracts in the medium, and at the concentration of 15 per cent, the wandering velocity of pseudoeosinophils is better, and the duration of wandering capacity is longest as well as the regressive degeneration of cells is least ; and therefore, the chick-embryo extracts at the concentration of 15 per cent is the optimal concentration for the medium of this bone-marrow tissue culture. 2. The first diluted solution from the sediment of the chick-embryo from which extracts taken likewise affects the tissue culture almost in the same way as the chick-embryo extracts; namely, the relative tissue growth rate increases in a direct proportion to the concentration, and at the concentration of 20 per cent the wandering velocity of pseudoeosinophils is better and also the regressive degeneration is least. Therefore, the optimal concentration in the medium of the first diluted solution from the sediment is at 20 per cent. 3. As for the comparative effects of the 15% chick-embryo extracts against the 20% diluted solution from the sediment, the former surpasses the latter in every respects, namely, in the relative tissue growth rate, the wandering velocity of pseudoeosinophils and the lowness in the appearance of regressively degenerated granules. Consequently it is thought that the 15% chick-embryo extracts is the most suitable culture medium for the rabbit bone-marrow tissue culture by the roller-tube method.
ISSN
0030-1558
NCID
AN00032489