Journal of Okayama Medical Association
Published by Okayama Medical Association

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生体に於けるHem蛋白の分解に関する研究 第1編 肝臓の生理的食塩水抽出液に依るPyridin-verdohaemichromogen並びにGlobin-verdohaemichromogenの生成

三上 純一郎 岡山大学医学部第一内科教室
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The reaction of pyridin-haematin or hemoglobin to the liver extract with physiologic saline solution was observed under various conditions. The results were as follows. 1. The formation of pyridin-verdohaemichromogen from pyridin-haematin was most suitable on the pH 8 of the hydrogen ion density of the reagent on the occasion of installation at 38℃., and the maximum value was observed in the 4th hour. 2. The formation of pyridin-verdohaemichromogen from pysidin-haemin was rather promoted on the pH 8 of the reagent on the occasion of installation at 60℃. than at 38℃., and the maximum value was also accelerated a little. 3. The formation of pyridin-verdohaemichromogen from pyridin-haematin was most powerful on the use of the liver extract of gunea pig among the liver extracts of gunia pig, white rat, rabbit and dog etc. on the occasion of the pH 8 and of installation at 38℃. 4. When pyridin-hematin and the liver extract of gunia pig were installated on the pH 8 at 33℃, the absorption curves of the ether extracts of the hourly reagents by the Engel's method displayed the absorption maximum at 655, 530 and 395 mμ, and the hight of those maximum was 655<530<395 mμ But the absorption maximum at 655 mμ was once elevated with hours until the 4th hour, then declined, and the maximum at 530 mμ was gradually declined without elevation and finally disadpeared, and the maximum at 395 mμ was also declined in some degree. Furthermore, a absorption maximum at 498 mμ was merely observed. 5. The formation of pyridinverdohaemichromogen was suppressed with the elevation of the extract's temperature and obstructed at 100℃. on the reaction between pyridin-haematin and the liver extract of guinea pig. 6. On the formation of globin-verdohaemichromogen on the occasion of installation at 38℃. under the pH 8, the absorption curve of the ether extract of the reagent by the Engel's method displayed the absorption maximum at 630, 540 and 395 mμ at the beginning after washing, and the hight of those maximum was 630<540<395 mμ. The absorption maxiumm at 630 mμ was once elevated with hours until the 16th hour and then declined, and the absorption maximum at 540 mμ was declined in some degree. 7. The absorption curve of the 0.5 % HCl extract after washing with ether and then water according to the Engel's method on the reagent at the 16th hour on the process of globin-verdohaemichromogen formation displayed the absorption maximum at 675 mμ and 366 mμ, and the hight of these absorption maximum was 675<366 mμ. It agreed with the absorption curve of biliverdin in acid solvent. And a light yellowish pigment was obtained from the extraction with chloroform in a weak acid condition after the reduction of the above product with natrium dithionit in weak basic condition, and it showed a positive diazo reaction on the addition of methanol after wahsing. 8. The absorption maximum (at 675 mμ) of the 0.5 % HCl extract solution of the hourly reagents by the Engel's method on the occasion of installation at 38℃. under the pH 8 on the globin-verdohaemichromogen formation by both haemoglobin and guinea pig liver extract were most high at the 16 hour and agreed with the vicissitude of the ether extract's absorption maximum at 630 mμ. 9. The formation of globin-verdohaemichromogen from haemoglobin was more promoted on the occasion of the installation at 60℃. than at 38℃. in the same pH 8. 10. When the guinea pig liver extract was heated on the reaction between haemoglobin and guinea pig liver extract, the formation of globin-verdohaemichromogen was suppressed with the elevation of temperature and obstructed at 100℃. 11. The reaction between haemoglobin and guinea pig liver extract was more promoted by an aeration of molecular oxygen than it was under an installation, but the obtained dosis of globin-verdohaemichromogen was decreased. 12. The katalase activity in the liver extract of guinea pig was rapidly declined with heat, and it was more effective on this occasion than it was effective to suppress on the globin-verdohaemichromogen formation. 13. Since the above results, it is supposed that pyridin-haematin or haemoglobin is analysed to bilirubin by the liver extracts and this process is perticipated by the enzym system.
ISSN
0030-1558
NCID
AN00032489