Similar to the previous report, 4 strains belonging to St. aureus and albus selected from the isolated bacteria, and each standard strain of aureus and albus and these bacteria passing through the animal were used as test bacteria. In the present experiment N-source requirement during the growth of these bacteria, and oxidation and production of amino acids by resting cells were studied; and glucose oxidation by growing bacteria and resting cells was compared. The results are as follows. 1. The N-source requirement in the case of albus compared with aureus in general tended to be greater but no significant difference could be recognized between standard strains and those passing through the animal or the isolated strains. 2. With respect to the oxidation and production of amino acids by resting cells, no distinct difference could be found either between standard strains and the bacteria passing through the animal or the isolated strains. 3. On comparing the glucose oxidation by growing bacteria and resting cells of each strain, during the process of glucose metabolism both the strains that passed through the animal and the isolated strains tend to show a weaker oxidation of pyruvate and of those that follow than the oxidation by our laboratory strains.