Journal of Okayama Medical Association
Published by Okayama Medical Association

Full-text articles are available 3 years after publication.


阪口 義彦 岡山大学大学院医歯学総合研究科病原細菌学
117_41.pdf 6.36 MB
Clostridium botulinum types C and D toxin production is governed by the specific bacteriophages. A type C phage,c-st,was isolated from a toxigenic strain of C-Stockholm,and its whole genome sequence was determined.The genome was a linear double-stranded 185,681bp DNA with a G+C content of 26.2%, and identical 403-bp sequences were found at each end of the genome. From the DNA research, 198 potential protein-coding regions (ORFs)were identified. The genes for type C progenitor toxin and C3 exoenzyme were identified, but no type D toxin gene was found.A number of genes were homologous to those of a Bacillus subtilis bacteriophage,SP D. On Southern blot analysis between type C and D cell DNAs and the specific probes for H and L chains of C and D neurotoxins, it became clear that type C and D strains posses only one type of toxin gene.From these findings, it is concluded that cross-neutralization observed between toxins (types C and D)and their antiserums can be caused by the common-antigens in C and D toxins.
ボツリヌス菌 (Clostridium botulinum)
ファージ (phage)
神経毒素遺伝子 (neurotoxin gene)