Journal of Okayama Medical Association
Published by Okayama Medical Association

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C型慢性肝炎におけるHCV-免疫複合体形成とインターフェロン治療効果との関係

眞鍋 良二 Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
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抄録
We retrospectively analyzed the relationship between formation of HCV-bearing immune complexes (HCV-IC) and response to interferon (IFN) therapy for chronic hepatitis C (CH-C). Five CH-C patients with complete response (CR) to IFN therapy and pre-treatment HCV RNA titer measured by Amplicor HCV-monitor and five stage (biopsy proved fibrosis of the liver)-matiched CH-C patients who were non-esponders (NR) to IFN therapy were enrolled. Pretretment sera were separated into HCV-IC (supernatant) and anti-HCV antibody-free virions (pellet) by addition of anti-human immunoglobulins and centrifugation. HCV RNA was quantified in the supernatant and the pellet, by real-time PCR and semi-quantitative RT-PCR respectively. There were discrepancies in the HCV RNA titers measured by Amplicor and the results of real-time PCR. We found a larges quantity of HCV RNA in the supernatan and a smaller quantity in the pellet in CR than in NR by using by real-time PCR. Our data indicate that response to interferon therapy for CH-C patients may be associated with the conditions underlying the formation of HCV-IC.
キーワード
HCV
chronic hepatitisC
HCV-immune complexes
備考
原著
ISSN
0030-1558
NCID
AN00032489