Journal of Okayama Medical Association
Published by Okayama Medical Association

<Availability>
Full-text articles are available 3 years after publication.

Mn-SOD および Cu/Zn-SOD 様免疫組織化学反応陽性細胞のラット脳内分布

王 燕 岡山大学医学部解剖学第三講座
Thumnail 110_9.pdf 2.82 MB
抄録
The localization of manganese (Mn) and copper-zinc (Cu/Zn) superoxide dismutase (SOD)-like immunoreactive (lir) cells in the normal rat brain and the change in numbers of both SOD-lir cortical cells after the injection of ferric chloride into the sensorimotor cortex were investigated immunohistochemically. Large and medium-sized cell bodies, pyramidal to polygonal in shape, were immunostained by anti-Mn-SOD antibody. Most of these were not immunolabeled by anti-GFAP antibody, suggesting that most of the Mn-SOD-lir cells are neurons. These cell bodies were distributed in some selected brain areas with different densities. The densest population of Mn-SOD-lir cells was found in the hypothalamic and limbic structures, and in some brain stem nuclei, such as the thalamic reticular nucleus, the locus ceruleus and the pontine nucleus. Anti-Cu/Zn-SOD antibody immunostained many small round cells throughout both white and gray matter, most of which were also immunolabeled by anti-GFAP antiserum. After intacortical injection of FeCl3, the numbers of Mn- and Cu/Zn-SOD-lir cells per unit area within the adjacent narrow region of the injection site significantly increased 2 and 6 hr after the injection, respectively.
キーワード
Mn-SOD
Cu/Zn-SOD
免疫組織化学
ラット脳内分布
FeCl3 大脳皮質内注入
備考
原著
ISSN
0030-1558
NCID
AN00032489