高速液体クロマトグラフィーによる中国野菜中のアスコルビン酸および総アスコルビン酸の定量

A Simple high-performance liquid chromatographic method is described for the rapid estimation of ascorbic acid and total ascorbic acid. Use of tetra-n-butylammonium bromide as an ion-pairing reagent in the mobile phase yielded reproducible retention for ascorbic acid. Total ascorbic acid is determined by reducing the dehydroascorbic acid to ascorbic acid by treatment with DL-homocysteine. This high-performance liquid chromatographic method has been applied to the analysis of several Chinese vegetables. These Chinese vegetables contained more total ascorbic acid than any other vegetables on the market.

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