Journal of Okayama Medical Association
Published by Okayama Medical Association

Full-text articles are available 3 years after publication.

DNA 分析による生物試料の性別判定に関する法医学的研究

中井 三代子 岡山大学医学部法医学教室
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Sex identification of human blood, bloodstains, saliva, hair roots and bone marrow was conducted by DNA analysis. The effects of various contaminants in samples on sex identification were studied, using 32-week-old contaminated bloodstains. The effect of heat or decomposition on sex identification was examined by using the blood obtained from autopsy-cases. A 2.12 kb band with male DNA, digested with Hae Ⅲ and electrophoresed, was observed by Southern hybridization using a human Y chromosome-specific probe, while a faint 2.00 kb band with female DNA was also sometimes detected. The male 2.12 kb band was detected even in 5-year-old bloodstains. The 2.12 kb band was also detected frequently in contaminated bloodstains as old as 32 weeks. The band was observed by Southern hybridization analysis in decomposed blood as old as 7 days, while it could not be detected in heated and decomposed blood after 3 days.
sex identification
deoxyribonucleic acid (DNA)
forensic science