Bulletin of Faculty of Health Sciences Okayama University Medical School
Published by Faculty of Health Sciences Okayama University Medical School

<Formerly known as>
岡山大学医療技術短期大学部紀要 (1巻-9巻)

Human serum lipoprotein-cyclodextrin interaction in agarose gel

一村 光子 岡山大学医療技術短期大学部衛生技術学科
唐下 博子 岡山大学医療技術短期大学部衛生技術学科
崎山 順子 岡山大学医療技術短期大学部衛生技術学科
森 秀治 岡山大学医療技術短期大学部衛生技術学科
伊藤 昔子 岡山大学医療技術短期大学部衛生技術学科
中田 安成 岡山大学医療技術短期大学部衛生技術学科
遠藤 浩 岡山大学医療技術短期大学部衛生技術学科
Cyclodextrins (CDs) are cyclic oligosaccharides with low molecular weight. They have been known to bind to some serum lipoproteins and to form complexes. To elucidate whether each serum lipoprotein subclass could be separated by electrophoresis using CDs, we performed preliminary experiment, in which lipoprotein-CDs interaction was examined on electrophoresis with agarose gel. When the supporting agarose gel containing both α-CD and β-CD was prepared and was applied to isoelectric focusing for fractionating serum lipoproteins, apoB lipoproteins were found to be clearly separated into several fractions on this electrophoresis. This finding suggested that apoB lipoprotein may be detected as isolated form by arranging amounts of added CDs.
血清apoB含有リポタンパク質 (serum apoB lipoprotein)
シクロデキストリン (cyclodextrin)
等電点電気泳動 (isoelectric focusing (IEF))
agarose gel