Bulletin of Faculty of Health Sciences Okayama University Medical School
Published by Faculty of Health Sciences Okayama University Medical School

<Formerly known as>
岡山大学医療技術短期大学部紀要 (1巻-9巻)

チオバルビツール酸(酢酸酸性)法によるミトコンドリアの二価鉄誘導脂質過酸化反応の測定

山本 剛禧 岡山大学医療技術短期大学部診療放射線技術学科
抄録
Mitochondrial lipid peroxidations were usually detected by the thiobarbituric acid (TBA) color reaction under the acidic conditions with hydrochloric and trichloroacetic acids. The time response curve of Fe(2+)-induced lipid peroxidation, accompanied the lag time of induction period, of mouse liver mitochondria was measured with detection of the produced lipid peroxides by the TBA method under the acidic conditions with acetic acid. Fe(2+)-induced lipid peroxidation was terminated by the addition of ethylenediaminetetraacetate (EDTA). The lag time of the peroxidation detected by TBA method in the acetic acid conditions was obtained as well as that detected in the hydrochloric and trichloroacetic acids conditions. The fact indicates that Fe(2+)-induced lipid peroxidation of mitochondria and its lipid peroxides are able to be measured by the TBA color reaction in the acetic acid conditions with EDTA.
キーワード
Mitochondrial Lipid Peroxidation
Thiobarbituric Acid
Ferrous Ion
ISSN
0917-4494
NCID
AN10355371