Bulletin of Faculty of Health Sciences Okayama University Medical School
Published by Faculty of Health Sciences Okayama University Medical School

<Formerly known as>
岡山大学医療技術短期大学部紀要 (1巻-9巻)

Cell cycle of tsAF8 after heating

澁谷 光一 岡山大学医学部保健学科放射線技術科学専攻
川崎 祥二 岡山大学医学部保健学科放射線技術科学専攻
黒田 昌宏 岡山大学医学部附属病院放射線科
浅海 淳一 岡山大学歯学部附属病院歯科放射線科
加藤 博和 岡山大学医学部保健学科放射線技術科学専攻
平木 祥夫 岡山大学医学部附属病院放射線科
抄録
Thermotolerance in tsAF8 cells develops during incubation at 34℃ after heating at 45℃, while it is suppressed by the following incubation at a non-permissive temperature of 39.7℃ after the same heating. The incubation temperature after heating may affect the cell cycle and consequently thermotolerance. In the present study, a relationship between the thermotolerance and the cell cycle of tsAF8 was investigated. The cell cycle fractions and DNA synthesis were measured by flow cytometry using double staining with propidium iodide and bromodeoxyuridine. When the tsAF8 cells were heated at 45℃ for 20 min, and thereafter incubated at 34℃, bromodeoxyuridine uptake in the S phase cells (DNA synthesis) was recovered to 65.1% 6 h after the heating, and the cells showed gradual accumulation in the G(2)/M phase. When the cells were incubated at 39.7℃ after heating at 45℃ for 20 min, then showed inhibition of thermotolerance development, the DNA synthesis was recovered to 15.1% temporarily 6 h after the heating, but it became 0% after 12 h, and the cells did not remarkably accumulate in any phases of the cell cycle. This inhibition of DNA synthesis at 39.7℃ was considered to be the result of cell survival decreasing by a step-down heating. However, the relationship between the thermotolerance and the cell cycle was not found out in tsAF8 cells, because the cells did not accumulate in any phases of the cell cycle under the inhibitory condition of thermotolerance.
キーワード
thermotolerance (温熱耐性)
hyperthermia (ハイパーサーミア)
tsAF8
cell cycle (細胞周期)
ISSN
1345-0948
NCID
AA11403004