Acta Medica Okayama 72巻 3号
2018-06 発行

A New Hepatitis Virus Test with Microliter-scale Fingertip Blood Collection in Japan

Namba, Shihoko Center for Innovative Clinical Medicine, Okayama University Hospital
Ikeda, Fusao Department of Gastroenterology and Hepatology,Okayama University Hospital
Takaguchi, Koichi Department of Internal Medicine, Kagawa Prefectural Central Hospital
Shimomura, Yasuyuki Department of Gastroenterology and Hepatology,Okayama University Hospital
Yasunaka, Tetsuya Department of Gastroenterology and Hepatology,Okayama University Hospital
Okada, Hiroyuki Department of Gastroenterology and Hepatology,Okayama University Hospital
Publication Date
2018-06
Abstract
We investigated whether a small amount of blood collected by fingertip blood sampling would be adequate in a mass examination for hepatitis virus infection in Japan. A cross-sectional survey was conducted at health fairs in Kasaoka City and Shodoshima Island, where participants took the hepatitis screening test. A total of 114 consecutive individuals who took the hepatitis screening test were enrolled. Twenty microliters of plasma was successfully obtained from all participants. Among the participants, two had positive results for HBs antigen and two were positive for anti-HCV; all four were > 60 years old and rarely visited the hospital. Thirty-three and 38 patients chronically infected with HBV and HCV, respectively, were examined for confirmatory assays at participating hospitals. All subjects with undetectable serum levels of HBs antigen and anti-HCV had undetectable levels of both markers in fingertip blood, and the levels in serum and fingertip blood were significantly correlated (p<0.01). The lower detection limit of HBs antigen was defined as 0.005 IU/ml, and the cut-off value of anti-HCV was 1.0 by using 10-μl fingertip blood samples. The fingertip blood sampling described herein may be adequate in mass examinations for hepatitis virus testing in Japan.
Document Type
Original Article
Keywords
fingertip
hepatitis test
HBV
HCV
Japan
Link to PubMed
Thumnail 72_3_283.pdf 2.28 MB