ID | 53976 |
フルテキストURL | |
著者 |
Nakagawa, Tomoyuki
Faculty of Applied Biological Science, Gifu University
Mitsui, Ryoji
Department of Biochemistry, Faculty of Science, Okayama University of Science
Tani, Akio
Institute of Plant Science and Resources, Okayama University
ORCID
Kaken ID
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Sasa, Kentaro
Faculty of Applied Biological Science, Gifu University
Tashiro, Shinya
Faculty of Applied Biological Science, Gifu University
Iwama, Tomonori
Faculty of Applied Biological Science, Gifu University
Hayakawa, Takashi
Faculty of Applied Biological Science, Gifu University
Kawai, Keiichi
Tokai Gakuin University
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抄録 | In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca2+-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. The genome of strain AM1 contains another MDH gene homologue, xoxF1, whose function in methanol metabolism has remained unclear. In this work, we show that XoxF1 also functions as an MDH and is La3+-dependent. Despite the absence of Ca2+ in the medium strain AM1 was able to grow on methanol in the presence of La3+. Addition of La3+ increased MDH activity but the addition had no effect on mxaF or xoxF1 expression level. We purified MDH from strain AM1 grown on methanol in the presence of La3+, and its N-terminal amino acid sequence corresponded to that of XoxF1. The enzyme contained La3+ as a cofactor. The ΔmxaF mutant strain could not grow on methanol in the presence of Ca2+, but was able to grow after supplementation with La3+. Taken together, these results show that XoxF1 participates in methanol metabolism as a La3+-dependent MDH in strain AM1.
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備考 | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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発行日 | 2013-03-22
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出版物タイトル |
PLOS ONE
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巻 | 7巻
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号 | 11号
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出版者 | PUBLIC LIBRARY SCIENCE
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開始ページ | e50480
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ISSN | 1932-6203
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1371/journal.pone.0050480
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言語 |
英語
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著作権者 | © 2012 Nakagawa et al.
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論文のバージョン | publisher
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査読 |
有り
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