ID 32220
JaLCDOI
フルテキストURL
著者
Yoshida, Iwao Osaka University
Takamizawa, Akihisa Osaka University
Fujita, Hiyoyuki Osaka University
Manabe, Sadao Osaka University
Okabe, Akinobu Kagawa Medical School
抄録

We constructed a plasmid, pBH103-ME5, in which the region encoding the 10 preS2 amino acid residues and the S domain of the hepatitis B surface antigen (HBsAg) were regulated by the promoter of the yeast repressible acid phosphatase gene. Saccharomyces cerevisiae carrying pBH103-ME5 produced the HBs antigen (yHBsAg), when it was cultured in a medium containing a low concentration of phosphate. The antigen was purified to homogeneity. Its molecular weight was determined by Western blotting to be 24,000, and its amino acid composition agreed well with that deduced from the nucleotide sequence. The C-terminal amino acid sequence of yHBsAg was exactly the same as that predicted from the nucleotide sequence, while the N-terminal amino acid acetylserine, which was followed by 8 amino acid residues coded by the preS2 region. These results indicate that the recombinant yeast produced a single polypeptide consisting of the preS2 region and the subsequent S domain after being processed at the N-terminus

キーワード
hepatitis B surface antigen
preS2 region
plasmid
yeast
Saccharomyces cerevisiae
Amo Type
Article
発行日
1991-02
出版物タイトル
Acta Medica Okayama
45巻
1号
出版者
Okayama University Medical School
開始ページ
1
終了ページ
10
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
査読
有り
Submission Path
amo/vol45/iss1/1
PubMed ID
Web of Sience KeyUT
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