ID 32209
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 1.15 MB
著者
Mukuzono, Hiroshi Okayama University
Yoshino, Tadashi Okayama University
Akagi, Tadaatsu Okayama University
抄録

A monoclonal antibody (MAb), OPT1, reactive with T cells in formalin-fixed, paraffin-embedded tissue sections, has been identified through immunization with activated T cells from peripheral blood lymphocytes (PBL). The antibody is an IgG1 antibody as demonstrated by the Ouchterlony technique. By cytofluorometric analysis, almost all CD3+ lymphocytes and only a few CD20+ lymphocytes of peripheral blood expressed the OPT1 antigen. Nonhematolymphoid cell lines were negative for OPT1 by the immunoperoxidase staining using acetone-fixed cell lines. On the contrary, peripheral T cells, cells of two T cell lines out of four and a part of the cells of one B cell line out of two were positive for OPT1. The immunoperoxidase staining of paraffin-embedded tissue sections revealed that most of lymphocytes in T cell areas of lymph nodes expressed OPT1 antigen. Some lymphocytes in both cortex and medulla of the thymus and erythroid precursors of the bone marrow were OPT1+. In the malignant lymphoma series, approximately 90% of T cell lymphomas and 6% of B cell lymphomas reacted with OPT1. None of the Reed-Sternberg cells nor Hodgkin cells in Hodgkin's disease were positive. Consequently, OPT1 may be useful for the diagnosis and study of malignant lymphomas and other related lesions.</P>

キーワード
monoclonal antibody
OPTI
T cells
lymphocytes
lymphoma
Amo Type
Article
発行日
1991-06
出版物タイトル
Acta Medica Okayama
45巻
3号
出版者
Okayama University Medical School
開始ページ
147
終了ページ
154
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
査読
有り
Submission Path
amo/vol45/iss3/4
PubMed ID
Web of Sience KeyUT
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