ID 32207
JaLCDOI
フルテキストURL
著者
Okada, Soji Okayama University
Ichiki, Ken Okayama University
Sato, Kimiaki Okayama University
Tanakuchi, So Okayama University
Ishii, Keita Okayama University
Hamada, Hiroshi Okayama University
Ota, Zensuke Okayama University
抄録

We studied the pathways of complement activation associated with the islet cell surface antibody (ICSA) obtained from sera of 7 patients (age less than 15 years) with insulin dependent diabetes mellitus (IDDM). The target cells were 51CR labelled rat islet cells and the complement source was human AB serum. Complement-dependent antibody mediated cytotoxicity (CAMC activity) was obtained using the percentage of cytotoxicity. CAMC activity of untreated sera was significantly inhibited by treating with EGTA or EDTA (p less than 0.001). The CAMC activity of EDTA-treated sera was significantly lower than that of EGTA-treated sera (p less than 0.001). In the inactivated human AB serum, it was lower than that of EGTA-treated sera (p less than 0.05), but not different from that of EDTA-treated sera. These results show that the complement activation associated with ICSA in patients occurred not only via the classical pathway but also via the alternative pathway.

キーワード
complement activation
islet surface antibody
insulin-dependent diabetes mellitus
complement-dependent antibody medeated cytotoxicity
classical pathway
alternative pathway
Amo Type
Article
発行日
1991-06
出版物タイトル
Acta Medica Okayama
45巻
3号
出版者
Okayama University Medical School
開始ページ
185
終了ページ
186
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
査読
有り
Submission Path
amo/vol45/iss3/9
PubMed ID
Web of Sience KeyUT
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