ID 31638
JaLCDOI
フルテキストURL
著者
Kuroda, Masahiro Okayama University
Inamura, Keiji Okayama University
Tahara, Seiji Okayama University
Kurabayashi, Yuzuru Okayama University
Akagi, Tadaatsu Okayama University
Asaumi, Junichi Okayama University
Togami, Izumi Okayama University
Takemoto, Mitsuhiro Okayama University
Honda, Osamu Okayama University
Morioka, Yasuki Okayama University
Kawasaki, Shoji Okayama University
Hiraki, Yoshio Okayama University
抄録

We developed a reliable system for the irradiation of xenografted tumors in mice which allows for accurate local irradiation under specific pathogen-free conditions. The system presented here consists of acrylic supports for mice and an acrylic box connected to a pump through 0.22 microns pore-sized filters. Mice with xenotransplanted tumors growing on their right hind legs were set on the supports and put into the box in a laminar flow hood. The tumors of 7 mice were irradiated simultaneously with X-rays of 6 and 10 MV generated by a linear accelerator at a dose rate of 3.1-4.7 Gy/min. The air was ventilated through filters during irradiation in the closed box. Microorganism tests confirmed that no bacteria entered or left the box. One of the significant characteristics of this setup is that it allows for irradiation under conditions of acute hypoxia, which is obtained using an integrated tourniquet. The dose variation among 7 tumors was less than 1%. The rest of the mouse's body was shielded effectively by a half-field technique and a lead block. As a result, the whole body dose for the mice was 0-4% of the total dose absorbed by the tumor. Due to the high dose rate and the ability to irradiate 7 mice simultaneously under specific pathogen-free conditions, this new system can be considered a time-saving and valuable tool for radiation oncology research.

キーワード
animal experiment
mouse
radiotherapy
linear accelerator
specirfic pathogen-free
Amo Type
Article
発行日
1999-06
出版物タイトル
Acta Medica Okayama
53巻
3号
出版者
Okayama University Medical School
開始ページ
111
終了ページ
118
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
Web of Sience KeyUT
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