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ID 17364
Eprint ID
17364
フルテキストURL
Thumnail 87_259.pdf 1.08 MB
タイトル(別表記)
The physicochemical studies on salt-resistant mechanism of Staphylococcus aureus
著者
片山 健 岡山大学医学部微生物学教室
抄録
To clarify salt-resistant mechanism of Staphylococcus aureus from the standpoint of the passive transport, attempts were made to establish the experimental system of artificial model membrane, liposome, and to measure permeability of Na(+) to it. The liposomes were prepared from phospholipids of S. aureus cultivated in normal and 10% NaCl media and NaCl-sensitive mutants. 1. In physiological conditions, it is possible to form the stable liposome comprised of phospholipids or total lipids of S. aureus. The preparation methods give a remarkable difference in the shape of vesicles. In the case of particles produced by hand or mechanical shaking, the diversities in the size and shape of individual particles can be observed. Sonicator-prepared liposomes are small and homogeneous of particle size, and consist of unilamella rather than multilamella. 2. Salt concentration affects liposome formation. Cardiolipin containing abundant branchedfatty acid form stable vesicles even in a high concentration of NaCl solution. 3. The liposome prepared from phospholipids of 10% NaCl S. aureus suppresses more markedly in the rate of Na(+) diffusion than that of normal S. aureus. The experiments on the liposomes prepared from fractionated individual phospholipids indicate that cardiolipin containing branchedfatty acid contributes to salt-resistance of S. aureus as a barrier. 4. From optical measurements, it has been elucidated that the liposome of phospholipids prepared from S. aureus acts as an ideal osmometer, and non-electrolyte, glycerol, shows a similar behavior to Na(+) in permeability.
発行日
1975-04-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
87巻
3-4号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
259
終了ページ
270
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/87/3-4/87_3-4_259/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma