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ID 15963
Eprint ID
15963
フルテキストURL
Thumnail 100_599.pdf 590 KB
タイトル(別表記)
Metabolic pathway of guanidinosuccinic acid biosynthesis
著者
松田 力哉 岡山大学医学部麻酔・蘇生学教室
抄録
In order to understand the metabolic abnormalities occurring with renal failure, the biosynthetic pathway of guanidinosuccinic acid (GSA) was investigated in normal rat liver, because GSA is known as a uremic toxin and its synthetic pathway is unclear.After fresh liver was homogenized and centrifuged for 60 min at 100,000×g, the supernatant was fractionated by precipitation with ammonium sulfate. It was found that the activity of GSA biosynthetic enzymes was highest in the fraction between 30% and 40% saturation of ammonium sulfate. The reaction mixture contained potassium phosphate buffer (pH7.4), aspartate, urea, ATP and an ATP-generating system, MnCl(2), and enzyme solution. The optimum pH was 7.4. The activity was strongly accelerated by Mn(++) and Cu(++), while it was completely inhibited by Fe(++). Hydroxyurea, L-canaline, L-canavanine, and L-arginine could not substitute for urea.These results suggest that GSA is not formed by the transamidination of arginine to aspartate as proposed by Cohen, or by way of the guanidine cycles proposed by Natelson. It is possible that GSA is mainly synthesized from urea and aspartate directly in rat liver.
キーワード
guanidinosuccinic acid
enzyme activity
metabolic pathway
urea
Guanidine Cycle
発行日
1988
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
100巻
5-6号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
599
終了ページ
608
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/100/5-6/100_5-6_599/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma