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ID 14234
Eprint ID
14234
フルテキストURL
Thumnail 103_859.pdf 3.63 MB
タイトル(別表記)
Persistent herpes simplex virus type 2 infection in a hamster kidney cell line with a characteristic to grow at a high culture temperature
著者
喜多村 哲朗 岡山大学医学部小児科学教室
抄録
A subline of the baby hamster kidney cell line (BHK-21), which was adapted to grow at 41℃ and named BHK-21H, was used to examine the effect of pre-treatment of host cells with a high culture temperature on the growth of herpes simplex virus (HSV). BHK-21H cells were infected with HSV type 2 (HSV-2) at 41℃ and maintained for 7 days at the same temperature. When these cells were further cultivated after shift-down to 36℃, persistent infections were established. These infections were characterized by a carrier culture state ; the infected cultures were composed of infected and non-infected cells, and cell destruction and regrowth of cells caused a balanced state of culture. Two to 3 months after the establishment of the persistent infections, virus production in the cultures stopped spontaneously and colonial regrowth of uninfected cells was observed. A subline (BHK-21R) which was cured of the persistent infection was obtained. Cells of this subline showed resistance to reinfection of HSV. Persistent HSV-2 Infection was also established by pre-treatment of BHK-21H cells at 41℃ before virus infection and maintenance of the infected cultures at 36℃. These findings indicate that establishment of the persistent HSV-2 infection is mainly attributable to reduced permissiveness of BHK-21H cells for virus growth caused after heat treatment of the cells.
キーワード
herpes simplex virus
persistent infection
supraoptimal temperature
carrier culture state
permissiveness
備考
原著
発行日
1991-08
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
103巻
7-8号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
859
終了ページ
867
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/103/7-8/103_7-8_859/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma