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ID 13864
Eprint ID
13864
フルテキストURL
Thumnail 106_1117.pdf 1.28 MB
タイトル(別表記)
Effect of isoflurane on energy status of isolated hepatocytes
著者
佐牟田 健 岡山大学医学部麻酔・蘇生学教室
抄録
We investigated the variation in the protection of energy status by isoflurane in isolated hepatocytes with the isoflurane dose, duration of anoxia, and reoxygenation by measurements of ATP, ADP, and AMP, in the cells. During 30 min of anoxia, in the presence of 1.4, 2.8, 4.2% isoflurane the ATP concentration was more than that with 0% isoflurane. With a 30-35 min incubation in the presence of 1.4% isoflurane, there was a modest decrease in energy charge during anoxia, partially prevented by isoflurane and completely reversed by reoxygenation, and no decrease in the total adenine nucleotide. With a 90-120 min incubation, isoflurane partly prevented the decreases in both energy charge and total adenine nucleotide during both anoxia and reoxygenation. We conclude that at doses in the clinical range, isoflurane partially protected isolated hepatocytes against decreases in both energy charge and total adenine nucleotide occurring either during short or long anoxia.
キーワード
イソフルラン
分離肝細胞
エネルギーチャージ
高エネルギーリン酸化合物
備考
原著
発行日
1994-12
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
106巻
11-12号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
1117
終了ページ
1124
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/106/11-12/106_11-12_1117/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
参考文献
1) Cohen PJ : Effect of anesthtic on mitochondrial function. Anesthesiology (1973) 39, 153-163.
2) Pathak BL, Becker GL, Reilly PJ, Hanson KA and Landers DF : Isoflurane partially preserves energy balance in isolated hepatocytes during in vitro anoxia. Anesth Analg (1991) 72, 571-577.
3) Samuta T, Becker GL, Pohorecki R, Armstrong K and Landers DF : Cyclosporine, isoflurane, and oxygenation effects on energy balance in isolated hepatocytes. Transplant Proc (1993) 25, 1847-1849.
4) Atkinson DE : The energy cahrge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry (1968) 7, 4030-4034.
5) Vincent MF, Van den Berghe G and Hers HG : The pathway of adenine nucleotide catabolism and its control in isolated rat hepatocytes subjected to anoxia. Biochem J (1982) 202, 117-123.
6) Frank AM, Ralf MW, Andries SK and Noordhoek J : Depletion of ATP but not of GSH affects viability of rat hepatocytes. Eur J Pahrmacol (1992) 228, 229-236.
7) Newberg LA and Michenfelder JD : Cerebral protection by isoflurane during hypoxemia or ischemia. Anesthesiology (1983) 59, 29-35.
8) Becker GL, Miletich DJ and Albrecht RF : Halogenated anesthetics increase oxygenconsumption in isolated hepatocytes from phenobarbital-treated rats. Anesthesiology (1987) 67, 185-190.
言語
Japanese
OAI-PMH Set
岡山大学
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Sort Key
2
Eprints Journal Name
joma