JaLCDOI 10.18926/46955
FullText URL mfe_38_1-2_091_096.pdf
Author Kanayama, Naoki| Yamakoshi, Kimi| Kiyomi, Masaaki| Magari, Masaki| Ohmori, Hitoshi|
Abstract Generally, IgM antibodies (Abs) produced in a primary immune response show lower affinity for an inducing antigen (Ag) compared with the corresponding IgG Abs that are major switched isotypes formed in the secondary response. An IgM molecule is a pentamer with 10 Ag-binding sites that will contribute to an increase of avidity for an Ag. To estimate the contribution of the pentameric structure to the avidity of an IgM Ab, we generated IgM and IgG1 monoclonal Abs (mAbs) with identical V regions that are specific for 4-hydroxy-3-nitrophenylacetyl (NP) by in vitro class switching of B cells followed by the cell fusion with a mouse myeloma cell line. Compared with an anti-NP IgG1 mAb, the corresponding IgM mAb showed much higher avidity for NP-conjugated bovine serum albumin, which was drastically reduced after being dissociated into monomers.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2004-03
Volume volume38
Issue issue1-2
Start Page 91
End Page 96
ISSN 0475-0071
language 英語
File Version publisher
NAID 80017001822
JaLCDOI 10.18926/47025
FullText URL mfe_36_2_051_060.pdf
Author Hikida, Masaki| Magari, Masaki| Nakayama, Yasunori| Kanayama, Naoki| Ohmori, Hitoshi|
Abstract A population of peripheral B cells have been shown to express recombination activating gene products, RAG-1 and RAG-2, which are considered to be involved in revising the B cell antigen receptor (BCR) in the periphery. BCR engagement has been reported to turn off RAG expression in peripheral B cells, whereas the same treatment has an opposite effect in immature B cells in the bone marrow. In contrast to receptor editing that is involved in the removal of autoreactivity in immature B cells, it has been shown that secondary V(D)J rearrangement in peripheral B cells, termed receptor revision, contributes to affinity maturation of antibodies. Here, we show that RAG-2 expression in murine splenic B cells was abrogated by the coligation of BCR with complement receptors (CD21/CD35) much more efficiently than by the engagement of BCR alone. On the other hand, the same coligation augmented proliferation of anti-CD40-stimulated B cells. Consistent with these observations, RAG-2 expression was lower in the draining lymph nodes of the quasi-monoclonal mice when they were immunized with a high-affinity antigen than with a low-affinity one. These findings suggest a crucial role for CD21/CD35 in directing the conservation or the revision of BCRs in peripheral B cells.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2002-03
Volume volume36
Issue issue2
Start Page 51
End Page 60
ISSN 0475-0071
language 英語
File Version publisher
NAID 80015582223
Author Faculty of Engineering, Okayama University|
Published Date 2012-01
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Volume volume46
Content Type Others
JaLCDOI 10.18926/19962
FullText URL Mem_Fac_Eng_OU_44_73.pdf
Author Imanaka, Hiroyuki| Tanaka, Soukichi| Feng, Bin| Imamura, Koreyoshi| Nakanishi, Kazuhiro|
Abstract We used modified Czapek-Dox (mCD) or dextrin-peptone-yeast extract (DPY) media to cultivate a filamentous fungus, Aspergillus oryzae IAM 2706 by three different cultivation methods, i.e., shaking-flask culture (SFC), agar-plate culture (APC), and membrane-surface liquid culture (MSLC), to identify the differences in cultivation behaviors and gene transcriptional profiles. The fungi cultivated by APC or MSLC secreted a greater number of different proteins/enzymes in larger quantities compared with fungi cultivated by SFC, particularly when DPY medium was used. In particular, the amounts of protease secreted by fungi cultivated via MSLC or APC were much greater compared with SFC. When mCD medium was used, α-amylase activity was barely detectable in all cultures while the activity was detected in MSLC and APC in a quantity that was several times higher than that in SFC using DPY medium. SDS-PAGE analysis and N-terminal amino acid sequences confirmed 6 proteins in the culture supernatants when DPY medium was used. Among these proteins oryzin (an alkaline protease) and α-amylase were detected at much higher levels in APC and MSLC compared with SFC, which was consistent with the measured activity of the secreted enzymes. However, when mCD medium was used, only oryzin was detected in significant amounts in MSLC and APC. Microarray analyses of the fungi cultivated by SFC, APC or MSLC using either mCD or DPY media indicated that the gene transcriptional profile of the MSLC sample was similar to that of the APC sample but different from that of the SFC sample. When mCD medium was used, most of the genes that were up-regulated 10-folds or greater in the MSLC sample relative to the SFC sample were unknown or predicted proteins. Transcription of the oryzin gene was only slightly up-regulated in the MSLC sample while transcription of the α-amylase gene was slightly down-regulated. On the other hand, when DPY medium was used, many known genes including the oryzin gene were up-regulated in the MSLC sample versus the SFC sample.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2010-01
Volume volume44
Start Page 73
End Page 83
ISSN 1349-6115
language 英語
File Version publisher
NAID 120002309053
Author Faculty of Engineering, Okayama University|
Published Date 2013-01
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Volume volume47
Content Type Others
JaLCDOI 10.18926/15391
FullText URL Mem_Fac_Eng_OU_27_1_33.pdf
Author Shimada, Yukiyasu| Suzuki, Kazuhiko| Sayama, Hayatoshi|
Abstract Neural nets have recently become the focus of much attention, largely because of their wide range of complex and nonlinear problems. This paper presents a new integrated approach using neural networks for diagnosing process failures. The fault propagation in process is modeled by causal relationships from the fault tree and its minimal cut sets. The measurement patterns required for training and testing the neural network were obtained from fault propagation model. The network is able to diagnose even in the presence of malfunction of certain sensors. We demonstrate via a nitric acid cooler process how the neural network can learn and successfully diagnose the faults.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1992-11-25
Volume volume27
Issue issue1
Start Page 33
End Page 44
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307902
JaLCDOI 10.18926/15414
FullText URL Mem_Fac_Eng_OU_26_1_27.pdf
Author Tomita, Eiji| Nsunge Felix Chintu| Hamamoto, Yoshisuke|
Abstract Distributions of hydrogen gas concentration in a suddenly started, single shot hydrogen gas jet issuing from a 1 mm diameter injector into still air were measured using laser interferometry method. This unsteady, turbulent free jet flow has also been calculated using the two-equation, high Reynolds number version of k-ε turbulence model and hybrid scheme for treating combined diffusion and convection in the SIMPLE algorithm. The injection pressure was 0.5 MPa for which predicted and measured temporal jet tip penetration distributions indicate that the jet discharged into still air at Mach 0.25. The level of agreement between present prediction and measurement is good in some regions and poor in others.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1991-11-30
Volume volume26
Issue issue1
Start Page 27
End Page 41
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307648
JaLCDOI 10.18926/15508
FullText URL Mem_Fac_Eng_OU_25_2_25.pdf
Author Nsunge Felix Chintu| Tomita, Eiji| Hamamoto, Yoshisuke|
Abstract A suddenly started cold methane gas jet issuing from a 1 mm diameter orifice into still air at Mach one has been predicted using the two-equation, high Reynolds number version of k-ε turbulence model and SIMPLE algorithm which employs so called primitive variables and a hybrid scheme for treating combined diffusion and convection. Global trends of predicted radial distributions of velocity, temperature, methane concentration in the steady rear part of the transient jet and axial jet tip penetration compare reasonably well with universal profiles representing measurement for the steady jet particularly in the fully developed turbulent core and semi-empirical relation for the transient jet respectively. The prediction scheme has shown reasonably good accuracy especially in prediction of main flow parameters of a transient, high speed compressible gas jet issuing into a dissimilar surrounding gas(binary gas mixture jet).
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1991-03-28
Volume volume25
Issue issue2
Start Page 25
End Page 38
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307676
JaLCDOI 10.18926/15510
FullText URL Mem_Fac_Eng_OU_25_2_39.pdf
Author Nsunge Felix Chintu| Tomita, Eiji| Hamamoto, Yoshisuke|
Abstract Velocity distributions and related parameters of transient and steady, turbulent air jets issuing under atmospheric conditions at Mach 0.14, 0.33 and 0.5 have been predicted using Navier-Stokes(N-S) equations for compressible flow and incompressible flow independently with the k-ε model. The closeness and consistence of the results predicted by the N-S equations for compressible and incompressible flows as well as with relevant measurement or similar prediction show that the incompressible flow assumption for at least some subsonic gas jets issuing at velocities higher than Mach 0.3, the general limit for incompressible fluid flow, can be reasonably accurate particularly in the main fully developed flow region. This suggests that the divergence term in source terms of the momentum, turbulence energy and its dissipation rate equations have negligible effects for some seemingly compressible high speed, subsonic free gas jets. The computation time is reduced by at least 18 % when incompressible flow assumption is used.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1991-03-28
Volume volume25
Issue issue2
Start Page 39
End Page 54
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307456
JaLCDOI 10.18926/15511
FullText URL Mem_Fac_Eng_OU_25_2_55.pdf
Author Ohkura Akira| Kameyama, Yoshimasa| Sayama, Hayatoshi| Suzuki, Kazuhiko| Fukumoto Shuichi|
Abstract AHP can handle decision-making problems involving several criteria when some of these are difficult or impossible to compare other than numerically. When a small group of decision makers settle the multi-criteria problem by AHP, the members of group could not often reach an agreement with hierarchic structure involving objectives, criteria, sub-criteria and alternatives because they have different positions, interests, and opinions. Further, the members have different importances for criteria and sub-criteria, and have dissimilar preferences for alternatives. In this article, we reveal the troubleness of AHP in case of being used by a small group of decision makers. Moreover, we proposed a procedure of AHP which the members of group could easily agree with the structure of problem and the weights of criteria etc.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1991-03-28
Volume volume25
Issue issue2
Start Page 55
End Page 67
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307741
JaLCDOI 10.18926/15438
FullText URL Mem_Fac_Eng_OU_28_1_61.pdf
Author Takeshita, Yuji| Kohno, Iichiro|
Abstract Knowledge of the unsaturated soil hydraulic properties is essential requirement for prediction of seepage flow and contaminant transport through the vadose zone. Unfortunately, these parameters are usually time consuming and expensive to measure in the field and laboratory. At the present condition, there are few data accumulation for Japanese soils. In this paper, van Genuchten's closed-form expressions are described to estimate unsaturated soil hydraulic properties. To evaluate the adequacy of these expressions, comparisons are performed between observed and calculated unsaturated hydraulic properties for typical Japanese soils.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1993-11-30
Volume volume28
Issue issue1
Start Page 61
End Page 69
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307801
JaLCDOI 10.18926/15451
FullText URL Mem_Fac_Eng_OU_22_45.pdf
Author Kohno, Iichiro| Nishigaki, Makoto| Takeshita, Yuji|
Abstract There are two classifications of the mechanism of levee failure caused by floods; local seepage failure and progressive failure. The fundamental causes of levee failure produced by piping and erosion were studied and the safety of river leves during floods evaluated in terms of soil machanics. The critical hydraulic gradient and the process of progressive failure were obtained from one- and two- dimensional model experiments for piping and erosion. Problems inherent in and preventive measures against levee failure are discussed. In particular, effects of the Tsukinowa method, the most representative Japanese flood fighting method, were studied experimentally and improvements proposed.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1988-03-31
Volume volume22
Start Page 45
End Page 62
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307536
JaLCDOI 10.18926/15483
FullText URL Mem_Fac_Eng_OU_24_1_107.pdf
Author Kohno, Iichiro| Nishigaki, Makoto| Takeshita, Yuji|
Abstract The numerical feasibility of determining soil water retention and hydraulic conductivity functions simultaneously from one-dimensional transient flow experiments in the laboratory by parameter estimation method is evaluated. Soil hydraulic properties are assumed to be represented by van Genuchten's closed-form expressions involving two unknown parameters: coefficients α and n . These parameters are evaluated by nonlinear least-squares fitting of predicted and observed pressure head with time. Gravity drainage experiments are performed for Toyoura standard sand to evaluate the adequacy of this proposed method.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1989-11-29
Volume volume24
Issue issue1
Start Page 107
End Page 114
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307749
JaLCDOI 10.18926/15498
FullText URL Mem_Fac_Eng_OU_25_1_99.pdf
Author Nishigaki, Makoto| Takeshita, Yuji| Kono, Iichiro|
Abstract In this paper, a numerical procedure of determining hydraulic properties in multilayered aquifers are presented. From pumping test data in multilayered aquifers, the coefficient of permeability and specific storage for each aquifer are determined by using a combination of finite element analysis and nonlinear least-squares optimization technique. This study especially points out necessity of stress-flow coupling analysis to explain the behaviors of pressure head in multilayered aquifer during pumping test. As a example, practical pumping test data were evaluate and the coefficients of permeability and specific storage of aquifers and aquitard were obtained.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 1990-12-14
Volume volume25
Issue issue1
Start Page 99
End Page 109
ISSN 0475-0071
language 英語
File Version publisher
NAID 120002307452
JaLCDOI 10.18926/19957
FullText URL Mem_Fac_Eng_OU_44_32.pdf
Author Kanatani, Kenichi| Niitsuma Hirotaka| Sugaya Yasuyuki|
Abstract We present an alternative approach to what we call the “standard optimization”, which minimizes a cost function by searching a parameter space. Instead, the input is “orthogonally projected” in the joint input space onto the manifold defined by the “consistency constraint”, which demands that any minimal subset of observations produce the same result. This approach avoids many difficulties encountered in the standard optimization. As typical examples, we apply it to line fitting and multiview triangulation. The latter produces a new algorithm far more efficient than existing methods. We also discuss optimality of our approach.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2010-01
Volume volume44
Start Page 32
End Page 41
ISSN 1349-6115
language 英語
File Version publisher
NAID 120002309124
JaLCDOI 10.18926/19959
FullText URL Mem_Fac_Eng_OU_44_50.pdf
Author Kanatani, Kenichi| Niitsuma Hirotaka| Rangrajan Prasanna|
Abstract We present highly accurate least-squares (LS) alternatives to the theoretically optimal maximum likelihood (ML) estimator for homographies between two images. Unlike ML, our estimators are non-iterative and yield solutions even in the presence of large noise. By rigorous error analysis, we derive a “hyperaccurate” estimator which is unbiased up to second order noise terms. Then, we introduce a computational simplification, which we call “Taubin approximation”, without incurring a loss in accuracy. We experimentally demonstrate that our estimators have accuracy surpassing the traditional LS estimator and comparable to the ML estimator.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2010-01
Volume volume44
Start Page 50
End Page 59
ISSN 1349-6115
language 英語
File Version publisher
NAID 120002308986
JaLCDOI 10.18926/44496
FullText URL mfe_045_015_026.pdf
Author Kanatani, Kenichi| Rangrajan, Prasanna| Sugaya, Yasuyuki| Niitsuma, Hirotaka|
Abstract We present a new least squares (LS) estimator, called “HyperLS”, specifically designed for parameter estimation in computer vision applications. It minimizes the algebraic distance under a special scale normalization, which is derived by rigorous error analysis in such a way that statistical bias is removed up to second order noise terms. Numerical experiments suggest that our HyperLS is far superior to the standard LS and comparable in accuracy to maximum likelihood (ML), which is known to produce highly accurate results in image applications but may fail to converge if poorly initialized. Our HyperLS is a perfect candidate for ML initialization. In addition, we discuss how image-based inference problems have different characteristics form conventional statistical applications, with a view to serving as a bridge between mathematicians and computer engineers.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2011-01
Volume volume45
Start Page 15
End Page 26
ISSN 1349-6115
language 英語
Copyright Holders Copyright © by the authors
File Version publisher
NAID 120002905952
JaLCDOI 10.18926/44498
FullText URL mfe_045_036_045.pdf
Author Kanatani, Kenichi| Niitsuma, Hirotaka|
Abstract We present a new method for optimally computing the 3-D rotation from two sets of 3-D data. Unlike 2-D data, the noise in 3-D data is inherently inhomogeneous and anisotropic, reflecting the characteristics of the 3-D sensing used. To cope with this, Ohta and Kanatani introduced a technique called “renormalization”. Following them, we represent a 3-D rotation in terms of a quaternion and compute an exact maximum likelihood solution using the FNS of Chojnacki et al. As an example, we consider 3-D data obtained by stereo vision and optimally compute the 3-D rotation by analyzing the noise characteristics of stereo reconstruction. We show that the widely used method is not suitable for 3-D data. We confirm that the renormalization of Ohta and Kanatani indeed computes almost an optimal solution and that, although the difference is small, the proposed method can compute an even better solution.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2011-01
Volume volume45
Start Page 36
End Page 45
ISSN 1349-6115
language 英語
Copyright Holders Copyright © by the authors
File Version publisher
NAID 80021759250
JaLCDOI 10.18926/48125
FullText URL mfe_046_001_009.pdf
Author Kanatani, Kenichi| Niitsuma, Hirotaka|
Abstract We optimally estimate the similarity (rotation, translation, and scale change) between two sets of 3-D data in the presence of inhomogeneous and anisotropic noise. Adopting the Lie algebra representation of the 3-D rotational change, we derive the Levenberg-Marquardt procedure for simultaneously optimizing the rotation, the translation, and the scale change. We test the performance of our method using simulated stereo data and real GPS geodetic sensing data. We conclude that the conventional method assuming homogeneous and isotropic noise is insufficient and that our simultaneous optimization scheme can produce an accurate solution.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2012-01
Volume volume46
Start Page 1
End Page 9
ISSN 1349-6115
language 英語
Copyright Holders Copyright © by the authors
File Version publisher
NAID 80022451620
JaLCDOI 10.18926/48127
FullText URL mfe_046_021_033.pdf
Author Kanatani, Kenichi| Niitsuma, Hirotaka|
Abstract Because 3-D data are acquired using 3-D sensing such as stereo vision and laser range finders, they have inhomogeneous and anisotropic noise. This paper studies optimal computation of the similarity (rotation, translation, and scale change) of such 3-D data. We first point out that the Gauss-Newton and the Gauss-Helmert methods, regarded as different techniques, have similar structures. We then combine them to define what we call the modified Gauss-Helmert method and do stereo vision simulation to show that it is superior to either of the two in convergence performance. Finally, we show an application to real GPS geodetic data and point out that the widely used homogeneous and isotropic noise model is insufficient and that GPS geodetic data are prone to numerical problems.
Publication Title Memoirs of the Faculty of Engineering, Okayama University
Published Date 2012-01
Volume volume46
Start Page 21
End Page 33
ISSN 1349-6115
language 英語
Copyright Holders Copyright © by the authors
File Version publisher
NAID 80022451622