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ID 49566
FullText URL
Author
Teshigawara, Sanae
Hida, Kazuyuki
Nakatsuka, Atsuko
Kanzaki, Motoko
Inoue, Kentaro
Terami, Takahiro
Katayama, Akihiro
Iseda, Izumi
Matsushita, Yuichi
Miyatake, Nobuyuki
McDonald, John F.
Hotta, Kikuko
Makino, Hirofumi
Abstract
Context: Vaspin is an adipokine with insulin-sensitizing effects identified from visceral adipose tissues of genetically obese rats. Objective: We investigated genetic and nongenetic factors that define serum concentrations of vaspin. Design, Setting and Participants: Vaspin levels were measured with RIA in Japanese subjects with normal fasting plasma glucose (NFG; n = 259) and type 2 diabetes patients (T2D; n = 275). Single nucleotide polymorphisms (SNP) at SERPINA12 (vaspin) gene locus were discovered, and five SNP were genotyped in the subjects with varied body mass index (n = 1138). Results: The level of serum vaspin in 93% of the samples was found to vary from 0.2 to nearly 2 ng/ml in NFG subjects (n = 259) and from 0.2 to nearly 3 ng/ml in T2D patients (n = 275) (Vaspin(Low) group), whereas a significant subpopulation (7%) in both groups displayed much higher levels of 10-40 ng/ml (Vaspin(High) group). In the Vaspin(Low) group, serum vaspin levels in T2D were significantly higher than healthy subjects (0.99 +/- 0.04 vs. 0.86 +/- 0.02 ng/ml; P < 0.01). Both in T2D and genotyped Japanese population, serum vaspin levels closely correlated with homeostasis model of assessment for insulin resistance rather than anthropometric parameters. By genotyping, rs77060950 tightly linked to serum vaspin levels, i.e. CC (0.6 +/- 0.4 ng/ml), CA (18.4 +/- 9.6 ng/ml), and AA (30.5 +/- 5.1 ng/ml) (P < 2 x 10(-16)). Putative GATA-2 and GATA-3 binding consensus site was found at rs77060950. Conclusions: Serum vaspin levels were related to insulin resistance, and higher levels of serum vaspin in 7% of the Japanese population are closely linked to minor allele sequence (A) of rs77060950. (J Clin Endocrinol Metab 97: E1202-E1207, 2012)
Published Date
2012-07
Publication Title
Journal of Clinical Endocrinology & Metabolism
Volume
volume97
Issue
issue7
Publisher
Endocrine Soc
Start Page
E1202
End Page
E1207
ISSN
0021-972X
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1210/jc.2011-3297
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/49133
language
英語
File Version
publisher
Refereed
True
DOI
Web of Sience KeyUT