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ID 49557
FullText URL
Author
Candan, Gerile
Ishikawa, Sanae
Fujimura, Atsushi
Hayashi, Keiichiro
Uneda, Atsuhito
Mori, Akiko
Matsui, Hideki
Tomizawa, Kazuhito
Abstract
Topical therapy is the most favored form of treatment for whitening against hyperpigmentation and sunburn because it lends itself to self-administration, patient compliance, and absence of systemic adverse effects. However, transdermal delivery of hydrophilic chemicals is difficult. The main purpose of this study is to develop a delivering system of hydrophilic drugs and proteins across the skin. Hydroquinone (HQ), a well-known tyrosinase inhibitor and antimelanogenesis compound, and enhanced green fluorescent protein (EGFP) were fused with eleven poly-arginine (11R). Both HQ-11R and EGFP-11R were efficiently delivered in B16 cells, a mouse melanoma cell line. HQ-11R was as effective as HQ alone at inhibiting melanin synthesis in B16 cells. EGFP-11R was efficiently delivered into cells of the epidermis with 4-(1-pyrenyl)-butyric acid (PB), a counteranion bearing an aromatic hydrophobic moiety, in vivo, but EGFP alone or EGFP-11R without PB was not. Finally, topical application of HQ-11R with PB significantly inhibited UV irradiation-induced pigmentation in guinea pigs compared with HQ alone. These results suggest that topical therapy using poly-arginine in combination with PB is useful for the delivery of hydrophilic drugs and proteins by the transdermal route.
Keywords
Transdermal delivery
Protein transduction
Poly-arginine
Tat
Hydroquinone
Tyrosinase inhibitor
Published Date
2012-09
Publication Title
Biomaterials
Volume
volume33
Issue
issue27
Publisher
Elsevier Ltd.
Start Page
6468
End Page
6475
ISSN
0142-9612
NCID
AA11522637
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1016/j.biomaterials.2012.04.056
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/49124
language
英語
Copyright Holders
(c) 2012 Elsevier Ltd. All rights reserved.
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Refereed
True
DOI
Web of Sience KeyUT