このエントリーをはてなブックマークに追加
ID 48570
FullText URL
Author
Nagai, Yusuke
Abstract
The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 +/- 1.5 times during 8 days of incubation; mean +/- SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 +/- 0.2 times; mean +/- SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture.
Keywords
Cell proliferation
Self-assembly
Hydrogel
Scaffold
Mechanical strain
Published Date
2012-02
Publication Title
Biomaterials
Volume
volume33
Issue
issue4
Publisher
Elsevier Ltd.
Start Page
1044
End Page
1051
ISSN
0142-9612
NCID
AA11522637
Content Type
Journal Article
Official Url
http://www.sciencedirect.com/science/article/pii/S0142961211012531
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/48434
language
英語
Copyright Holders
© 2011 Elsevier Ltd. All rights reserved.
File Version
author
Refereed
True
DOI
Web of Sience KeyUT