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ID 52814
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Author
Takasaki, Kazuto
Futo, Satoshi
Niwa, Kousuke
Kawase, Mitsuo
Akitake, Hiroto
Abstract
In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products.
Keywords
Cultivar discrimination
Multiplex PCR
Strawberry
Practical application
Retrotransposon
Published Date
2014-09-20
Publication Title
Journal of Biotechnology
Volume
volume185
Publisher
Elsevier B.V.
Start Page
57
End Page
62
ISSN
0168-1656
Content Type
Journal Article
language
英語
Copyright Holders
© 2014 The Authors. Published by Elsevier B.V.
File Version
publisher
Refereed
True
DOI