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ID 56683
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Chourashi, Rhishita Division of Biochemistry, National Institute of Cholera and Enteric Diseases
Mondal, Moumita Division of Biochemistry, National Institute of Cholera and Enteric Diseases
Sinha, Ritam Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Debnath, Anusuya Division of Biochemistry, National Institute of Cholera and Enteric Diseases
Das, Suman Division of Biochemistry, National Institute of Cholera and Enteric Diseases
Koley, Hemanta Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Sekhar Chatterjeea, Nabendu Division of Biochemistry, National Institute of Cholera and Enteric Diseases
Abstract
Vibrio cholera survival in an aquatic environment depends on chitin utilization pathway that requires two factors, chitin binding protein and chitinases. The chitinases and the chitin utilization pathway are regulated by a two-component sensor histidine kinase ChiS in V. cholerae. In recent studies these two factors are also shown to be involved in V. cholerae pathogenesis. However, the role played by their upstream regulator ChiS in pathogenesis is yet to be known. In this study, we investigated the activation of ChiS in presence of mucin and its functional role in pathogenesis. We found ChiS is activated in mucin supplemented media. The isogenic chiS mutant (ChiS-) showed less growth compared to the wild type strain (ChiS+) in the presence of mucin supplemented media. The ChiS- strain also showed highly retarded motility as well as mucin layer penetration in vitro. Our result also showed that ChiS was important for adherence and survival in HT-29 cell. These observations indicate that ChiS is activated in presence of intestinal mucin and subsequently switch on the chitin utilization pathway. In animal models, our results also supported the in vitro observation. We found reduced fluid accumulation and colonization during infection with ChiS- strain. We also found ChiS- mutant with reduced expression of ctxA, toxT and tcpA. The cumulative effect of these events made V. cholerae ChiS- strain hypovirulent. Hence, we propose that ChiS plays a vital role in V. cholerae pathogenesis.
Keywords
ChiS
Mucin
Vibrio cholerae
Virulence
Note
This is an Accepted Manuscript of an article published by Elsevier GmbH
Published Date
2016-12
Publication Title
International Journal of Medical Microbiology
Volume
volume306
Issue
issue8
Publisher
ELSEVIER GMBH
Start Page
657
End Page
665
ISSN
14384221
NCID
AA11427330
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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Web of Sience KeyUT
Related Url
isVersionf https://doi.org/10.1016/j.ijmm.2016.09.003
Project
Collaborative Research of Okayama University for Infectious Diseases in India