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ID 57452
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Abe, Fumitaka Division of Wheat and Barley Research, Institute of Crop Science, NARO
Haque, Emdadul Division of Wheat and Barley Research, Institute of Crop Science, NARO
Hisano, Hiroshi Institute of Plant Science and Resources, Okayama University
Tanaka, Tsuyoshi Division of Basic Research, Institute of Crop Science, NARO
Kamiya, Yoko Kihara Institute for Biological Research, Yokohama City University
Mikami, Masafumi Graduate School of Nanobioscience, Yokohama City University
Kawaura, Kanako Kihara Institute for Biological Research, Yokohama City University
Endo, Masaki Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
Onishi, Kazumitsu Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine
Hayashi, Takeshi Division of Basic Research, Institute of Crop Science, NARO
Sato, Kazuhiro Institute of Plant Science and Resources, Okayama University Kakenhi
Abstract
1Common wheat has three sets of sub-genomes, making mutations difficult to observe, especially for traits controlled by recessive genes. Here, we produced hexaploid wheat lines with loss of function of homeoalleles of Qsd1, which controls seed dormancy in barley, by Agrobacterium-mediated CRISPR/Cas9. Of the eight transformed wheat events produced, three independent events carrying multiple mutations in wheat Qsd1 homeoalleles were obtained. Notably, one line had mutations in every homeoallele. We crossed this plant with wild-type cultivar Fielder to generate a transgene-free triple-recessive mutant, as revealed by Mendelian segregation. The mutant showed a significantly longer seed dormancy period than wild-type, which may result in reduced pre-harvest sprouting of grains on spikes. PCR, southern blotting, and whole-genome shotgun sequencing revealed that this segregant lacked transgenes in its genomic sequence. This technique serves as a model for trait improvement in wheat, particularly for genetically recessive traits, based on locus information from diploid barley.
Keywords
CRISPR/Cas9
Qsd1
multiple mutation
seed dormancy
wheat
Published Date
2019-07-30
Publication Title
Cell Reports
Volume
volume28
Issue
issue5
Publisher
Cell Press
Start Page
1362
End Page
1369
ISSN
22111247
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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publisher
PubMed ID
DOI
Related Url
isVersionOf https://doi.org/10.1016/j.celrep.2019.06.090
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Fumitaka Abe, Emdadul Haque, Hiroshi Hisano, Tsuyoshi Tanaka, Yoko Kamiya, Masafumi Mikami, Kanako Kawaura, Masaki Endo, Kazumitsu Onishi, Takeshi Hayashi, Kazuhiro Sato, Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat, Cell Reports, Volume 28, Issue 5, 2019,