このエントリーをはてなブックマークに追加
ID 55415
FullText URL
Thumnail fig.pdf 350 KB
Author
Horihata, Kei Laboratory of Reproductive Physiology, Faculty of Agriculture, Okayama University
Yoshioka, Shin Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Sano, Masahiro Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Yamamoto, Yuki Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Kimura, Koji Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Skarzynski, Dariusz J. Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences
Okuda, Kiyoshi Laboratory of Reproductive Physiology, Faculty of Agriculture, Okayama University
Abstract
The corpus luteum (CL) synthesises and secretes progesterone (P4), which is essential for the establishment and maintenance of pregnancy in mammals. P4 is synthesised from cholesterol. Cholesterol is internalised by low-density lipoprotein receptor (LDLR) and/or scavenger receptor B1 (SR-BI), and is effluxed by ATP-binding cassette (ABC) transporter A1 (ABCA1) and G1 (ABCG1). To test the hypothesis that lipoprotein receptors and ABC transporters are involved in functional luteolysis, we examined the expression of LDLR, SR-BI, ABCA1 and ABCG1 in bovine CL during the luteal stages and after injection of prostaglandin (PG) F2α on Day 10 after ovulation. Expression of LDLR and SR-BI mRNA and protein was lower in the regressed luteal than late luteal stage. Injection of cows with a PGF2α did not affect LDLR mRNA and protein levels in the CL. Although expression of SR-BI mRNA did not change, SR-BI protein expression decreased 12 and 24 h after PGF2α injection. The overall findings of the present study suggest that the decreased expression of SR-BI induced by PGF2α is one of the factors responsible for the continuous decrease in P4 production during functional luteolysis.
Keywords
luteal phase
ovary
progesterone
prostaglandin
reproduction
Note
This is an Accepted Manuscript of an article published by CSIRO Publishing
Published Date
2016-05-17
Publication Title
Reproduction, Fertility and Development
Volume
volume29
Issue
issue7
Publisher
CSIRO Publishing
Start Page
1280
End Page
1286
ISSN
10313613
NCID
AA10718189
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
File Version
author
PubMed ID
DOI
Web of Sience KeyUT
Related Url
https://doi.org/10.1071/RD15538