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ID 55410
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Yamamoto, Yuki Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Kohka, Misa Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Kobayashi, Yoshihiko Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Woclawek-Potocka, Izabela Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences
Okuda, Kiyoshi Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Abstract
Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes (ECEs) and EDN receptors (EDNRs) were investigated. All isoforms of EDN (EDN1-3), ECE (ECE1 and ECE2) and EDNR (EDNRA and EDNRB) were immunolocalised in the epithelial cells of the ampulla and the isthmus. EDNRs were also immunolocalised in smooth-muscle cells. The mRNA expression of EDN2 and ECE2 was higher in cultured ampullary oviductal epithelial cells than in isthmic cells. The expression of EDN1, EDN2 and ECE2 in the ampullary tissue was highest on the day of ovulation. Oestradiol-17β increased EDN2 and ECE1 expression, while progesterone increased only ECE1 expression in cultured ampullary epithelial cells. These results indicate that EDNs are produced by epithelial cells and their target site is smooth-muscle and epithelial cells, and suggest that ovarian steroids are regulators of endothelin synthesis in ampullary oviductal epithelial cells.
Keywords
endothelin converting enzyme
endothelin receptor
epithelial cell
ovarian steroids
oviductal contraction and relaxation
Note
This is an Accepted Manuscript of an article published by CSIRO Publishing
Published Date
2014-11
Publication Title
Reproduction, Fertility and Development
Volume
volume28
Issue
issue6
Publisher
CSIRO Publishing
Start Page
673
End Page
681
ISSN
1031-3613
NCID
AA10718189
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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isVersionOf https://doi.org/10.1071/RD14076