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ID 55384
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Bui, Tra M. T. Department of Animal Science, Graduate School of Environmental and Life Sciences, Okayama University
Nguyễn, Khánh X. Department of Animal Science, Graduate School of Environmental and Life Sciences, Okayama University
Karata, Asako Department of Animal Science, Graduate School of Environmental and Life Sciences, Okayama University
Ferré, Pilar Department of Animal Science, Graduate School of Environmental and Life Sciences, Okayama University
Trần, Minh T. Department of Animal Science, Graduate School of Environmental and Life Sciences, Okayama University
Wakai, Takuya Department of Animal Science, Graduate School of Environmental and Life Sciences, Okayama University
Funahashi, Hiroaki Department of Animal Science, Graduate School of Environmental and Life Sciences, Okayama University
Abstract
The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5-3mm diameter). When cumulus-oocyte complexes (COCs) from medium-sized follicles (MF; 3-6mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200ngmL-1 VEGF during the first 20h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200ngmL-1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200ngmL-1 during the first 20h of IVM.
Keywords
pig
Note
This is an Accepted Manuscript of an article published by CSIRO Publishing
Published Date
2016-12-12
Publication Title
Reproduction, Fertility and Development
Volume
volume29
Issue
issue10
Publisher
CSIRO Publishing
Start Page
1902
End Page
1909
ISSN
1031-3613
NCID
AA10718189
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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Related Url
https://doi.org/10.1071/RD16321